COMBINED FISH PAINTING AND HARLEQUIN STAINING FOR CELL CYCLE-CONTROLLED CHROMOSOME ANALYSIS IN HUMAN-LYMPHOCYTES

Authors
KULKA U HUBER R MULLER P KNEHR S BAUCHINGER M
Citation
U. Kulka et al., COMBINED FISH PAINTING AND HARLEQUIN STAINING FOR CELL CYCLE-CONTROLLED CHROMOSOME ANALYSIS IN HUMAN-LYMPHOCYTES, International journal of radiation biology, 68(1), 1995, pp. 25-27
Citations number
12
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
Journal title
International journal of radiation biology → ACNP
ISSN journal
09553002
Volume
68
Issue
1
Year of publication
1995
Pages
25 - 27
Database
ISI
SICI code
0955-3002(1995)68:1<25:CFPAHS>2.0.ZU;2-K
Abstract
We present a simple method that allows scoring of FISH-painted chromos omes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a diff erential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromo some damage for human biodosimetry.