COMPARATIVE-ANALYSIS OF SOME ISOZYMES AND PROTEINS IN SOMATIC AND ZYGOTIC EMBRYOS OF SOYBEAN (GLYCINE MAX[L] MERR)

Isozyme systems of peroxidase (E.C. 1.11.1.7), esterase (3.1.1.2), aci d phosphatase (E.C. 3.1.3.2) and total protein composition have been s tudied in soybean somatic embryos that were induced on immature zygoti c embryos or were maintained as long-term (repetitive) embryogenic cul ture on MSB-medium with 10 mu M 2,4-D. Protein and isozyme profiles we re compared to those of zygotic embryos and seeds of soybean. Peroxida se isozyme spectra found in callus and in somatic embryos were much mo re diversified in contrast to low peroxidase activity and poor isopero xidase spectrum in zygotic embryos. Isoesterase as well as acid phosph atase profiles of the somatic embryos are very similar to those of zyg otic embryos, isoesterase spectrum of the callus differs qualitatively from the spectrum of the somatic embryos. Some typical soybean seed p roteins (subunits of storage proteins, trypsin inhibitor) are synthesi zed in the somatic embryos.