SCOPOLETIN-GLUCOSYLTRANSFERASE ACTIVITY FROM DUBOISIA MYOPOROIDES - IMPROVEMENT OF CULTIVATION CONDITIONS AND CRUDE EXTRACT PREPARATION PROCEDURE

In order to prepare an active enzyme extract of UDP-glucose: scopoleti n glucosyltransferase from suspension cultured cells of Solanaceous sp ecies, the analytical conditions were improved, extraction of crude en zyme optimized, and cells of Duboisia myoporoides, Nicotiana plumbagin ifolia, and Solanum aviculare were compared as sources. Cells of Duboi sia gave best yields, which could be further increased by precultivati on in the presence of phenobarbital. For extraction of crude enzyme, T ris and phosphate buffers were compared, with addition of mercaptoetha nol, EDTA and ascorbate. The activity of SGTase exhibited 14.10(-3) nK at. mg(-1) of proteins using 130 mu M of scopoletin as a substrate.