Jb. Christensen et al., THE EFFECTS OF IN-USE SURGICAL HANDWASHING ON THE PREOPERATIVE AND POSTOPERATIVE FINGERTIP FLORA DURING CARDIOTHORACIC AND ORTHOPEDIC-SURGERY, The Journal of hospital infection, 30(4), 1995, pp. 283-293
Two operating teams (25 persons) were followed for two months with fin
gerprint samples taken preoperatively; before and after 'in-use' surgi
cal handwashing; and immediately postoperatively, with and without sur
gical gloves. The mean time for handwashing for the cardiothoracic tea
m (CT) was 2 min and for the orthopaedic team (OT) was 3.5 min. A clos
er observation of 10 persons revealed a great individual variation in
washing techniques, in spite of standard guidelines. The CT team perfo
rmed eight, and the OT team nine sterile operations with an average du
ration of 3 h and 20 min and 2 h and 40 min, respectively. Surgical ha
ndwashing resulted in fingertip sterility in 111/118 (94.1%) cases; in
61/66 (92.4%) samples from the surgeons and in 50/52 (96.2%) samples
from the assistants. Postoperative fingerprinting with gloves on showe
d sterile conditions in 85/91 (93.4%) samples; 57/59 (96.6%) from the
surgeons and 28/32 (87.5%) from the assistants. Immediately after remo
val of the gloves, 43/67 (64.2%) of fingerprint samples from the surge
ons and 13/48 (27.1%) from the assistants were still sterile. Coagulas
e-negative staphylococci (CNS) and Bacillus species predominated in fi
ngerprint samples. Of the 105 CNS strains tested, 11.4% were methicill
in resistant. Only five strains of Staphylococcus aureus were isolated
; in 4/5 cases from the OT. This study illustrates that in spite of st
andard guidelines, there is great individual variation in surgical han
dwashing. However, in most instances, the bacteria are eradicated from
the fingertips. Even after surgery for 2-3 h, there may still be a re
sidual effect of the hand disinfecting agent in half of the cases.