SEQUENCES WITHIN THE PARVOVIRUS MINUTE VIRUS OF MICE NS2-SPECIFIC EXON ARE REQUIRED FOR INCLUSION OF THIS EXON INTO SPLICED STEADY-STATE RNA

When the minute virus of mice NS2-specific exon was modified by either substitution or deletion, most P4-generated pre-mRNA was spliced from the large-intron donor at nucleotide 514 to the small-intron acceptor at nucleotide 2377. Improvement to consensus of large-intron splice s ites in such mutants did not suppress exon skipping or restore large i ntron excision. Therefore, sequences within the NS2-specific exon are required for inclusion of this exon into spliced, steady-state minute virus of mice RNA.