ISOLATION OF NOVEL HUMAN ENDOGENOUS RETROVIRUS-LIKE ELEMENTS WITH FOAMY VIRUS-RELATED POL SEQUENCE

Citation
A. Cordonnier et al., ISOLATION OF NOVEL HUMAN ENDOGENOUS RETROVIRUS-LIKE ELEMENTS WITH FOAMY VIRUS-RELATED POL SEQUENCE, Journal of virology, 69(9), 1995, pp. 5890-5897
Citations number
58
Categorie Soggetti
Virology
Journal title
ISSN journal
0022538X
Volume
69
Issue
9
Year of publication
1995
Pages
5890 - 5897
Database
ISI
SICI code
0022-538X(1995)69:9<5890:IONHER>2.0.ZU;2-2
Abstract
A new class of reverse transcriptase coding sequences was detected in reverse transcribed RNAs from human placenta by polymerase chain ampli fication with primers in highly conserved regions of the pol gene of m ammalian retroviruses and retrotransposons. Using one of these novel s equences as a probe to screen a human genomic library, we isolated ret rovirus-like elements bordered by long terminal repeats and having a p otential leucine tRNA primer-binding site. Determination of the comple te nucleotide sequence (6,591 bp) of one of these elements, termed HER V-L (for human endogenous retrovirus with leucine tRNA primer), reveal ed domains of amino acid similarities to retroviral reverse transcript ase and integrase proteins. In addition, a region with homologies to d UTPase proteins was found unexpectedly downstream from the integrase d omain. Amino acid sequence and phylogenetic analyses indicate that the HERV-L pol gene is related to that of foam;g retroviruses. HERV-L-rel ated sequences are detected in several mammalian species and have expa nded in primate and mouse genomes up to 100 to 200 copies.