EVALUATION OF THE AUTHENTICITY OF HALOARCHAEAL STRAINS BY RANDOM-AMPLIFIED POLYMORPHIC DNA

Authors
MARTINEZMURCIA AJ BOAN IF RODRIGUEZVALERA F
Citation
Aj. Martinezmurcia et al., EVALUATION OF THE AUTHENTICITY OF HALOARCHAEAL STRAINS BY RANDOM-AMPLIFIED POLYMORPHIC DNA, Letters in applied microbiology, 21(2), 1995, pp. 106-108
Citations number
10
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
21
Issue
2
Year of publication
1995
Pages
106 - 108
Database
ISI
SICI code
0266-8254(1995)21:2<106:EOTAOH>2.0.ZU;2-Z
Abstract
PCR analysis was performed on several archaeal species using 11 differ ent 10-mer oligonucleotides of arbitrary sequence. Duplicates of the t ype strain of Halococcus morrhuae (ATCC 17082 and CCM 537) showed mark edly different RAPD profiles. Moreover, RAPD patterns from the type st rain of Haloferax denitrificans ATCC 35960 and the strain ATCC 49116 d eposited as Haloferax larsenii', were identical. This method represent s an invaluable instrument in microbial screening and can clarify prob lems with strain identities.