VALIDATION OF 2 IMMUNOASSAY METHODS FOR ENVIRONMENTAL MONITORING OF CARBARYL AND 1-NAPHTHOL IN-GROUND WATER SAMPLES

Two formats (microtiter plates and magnetic particles) of sensitive en zyme-linked immunosorbent assays (ELISA) for carbaryl determination ha ve been compared to the EPA method 531.1 (liquid chromatography-post-c olumn derivatization-fluorescence detection, LC-PCR-FD). An immunoassa y developed for 1-naphthol determination has also been evaluated to mo nitor previous exposure to carbaryl in natural water samples. Matrix e ffect of this kind of water on the immunoassays is negligible and no s ample preparation has found to be necessary other than buffering the s amples. It is possible to perform a high number of analyses in a short period of time. A close correspondence was found for the results obta ined when spiked and well water samples were split for analysis by ELI SA and by LC-PCR-FD. Both methods are compared in terms of precision, reliability, reproducibility and their utility as screening tools. App lication of those methods to the direct determination of carbaryl and 1-naphthol in ground water samples of the aquifer Campo de Nijar (Alme ria, Spain) is reported.