ENZYME IMMUNOASSAYS FOR S-TRIAZINE HERBICIDES AND THEIR APPLICATION IN ENVIRONMENTAL AND FOOD ANALYSIS

Citation
M. Franek et al., ENZYME IMMUNOASSAYS FOR S-TRIAZINE HERBICIDES AND THEIR APPLICATION IN ENVIRONMENTAL AND FOOD ANALYSIS, Analytica chimica acta, 311(3), 1995, pp. 349-356
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
311
Issue
3
Year of publication
1995
Pages
349 - 356
Database
ISI
SICI code
0003-2670(1995)311:3<349:EIFSHA>2.0.ZU;2-4
Abstract
Seventeen rabbits and forty mice were immunized with immunogens prepar ed by derivatizing atrazine and simazine and conjugating them via amin ohexanecarboxylic acid and thiopropanecarboxylic acid substituents to proteins. Six rabbit antisera and one murine monoclonal antibody were incorporated into the ELISA (enzyme linked immunosorbent assay) format using peroxidase and alkaline phosphatase tracers. Superior antisera for atrazine, simazine and ametryn exhibited in optimized assays a 50% binding inhibition at 0.16, 0.25 and 0.45 mu g l(-1), respectively. T he IC50 value for the monoclonal antibody to atrazine was about 1.0 mu g l(-1). The most sensitive antiserum Atr-(CH2)(5)-OV-3C exhibited 74 .1% cross-reactivity with propazine and 26.3% with deethylatrazine. An tisera produced in response to the simazine-S-(CH2)(2)-determinant sho wed a remarkable variety in s-triazine recognition selectivity. Antise rum Sim-S-(CH2)(2)-KLH-C was extremely sensitive to ametryn, terbutryn and prometryn with cross-reactivities based on simazine (= 100%) 2200 , 1100 and 1480%, respectively. Structural aspects of these findings a re discussed. ELISA for atrazine was evaluated on surface water and gr ound water samples collected from a contaminated area. The amounts of atrazine found by ELISA were in good agreement with GC-MS analysis. St arting data for the application of ELISA to juice and milk samples are also presented.