Df. Akintola et al., DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HUMAN METALLOTHIONEIN-1 IN PLASMA AND URINE, The Journal of laboratory and clinical medicine, 126(2), 1995, pp. 119-127
Citations number
33
Categorie Soggetti
Medical Laboratory Technology","Medicine, General & Internal
The development of a sensitive enzyme-linked immunosorbent assay (ELIS
A) for human metallothionein-1 is reported. Metallothionein was purifi
ed from postmortem human liver and used to raise high-titer antibodies
in rabbits. The assay was specific for human metallothionein-1 (MT-1)
, and there was no significant cross-reaction with human metallothione
in-2. The detection limit (sensitivity) of the assay was 5 ng/ml, and
the added MT-1 could be fully recovered from plasma and urine. The nor
mal reference range for MT-1 was 32 +/- 16 ng/ml in plasma and 10 +/-
6 ng MT-1 per micromole of creatinine in random samples of urine. No s
ignificant differences were found between the values for males and fem
ales. The concentration of MT-1 was greatly increased between 24 and 4
8 hours otter surgery, indicating that the protein behaves like an acu
te phase reactant in human subjects.