P. Scarpellini et al., NESTED POLYMERASE CHAIN-REACTION FOR DIAGNOSIS AND MONITORING TREATMENT RESPONSE IN AIDS PATIENTS WITH TUBERCULOUS MENINGITIS, AIDS, 9(8), 1995, pp. 895-900
Objective: To investigate the usefulness of polymerase chain reaction
(PCR) from cerebrospinal fluid (CSF) for rapid diagnosis and assessing
treatment response of tuberculous meningitis (TBM) in AIDS patients.
Patients: Forty-four CSF samples from 10 patients with TBM confirmed b
y autopsy or by a culture of CSF (41 samples) and from two patients wi
th highly probable TBM (three samples) were analysed. CSF specimens we
re collected before and during standard antituberculous treatment. CSF
samples from 24 AIDS patients with autopsy evidence of other neurolog
ic diseases were studied as controls. Methods: A nested PCR amplifying
a 123 base-pair fragment of the IS6110 sequence was developed. Heatin
g to 95 degrees C for 15 min was used for pre-PCR treatment of samples
. Results: Detection limit was 10(2) colony-forming units per ml or 10
fg purified Mycobacterium tuberculosis DNA. M. tuberculosis DNA was d
etected in CSF from all the 12 confirmed or highly probable TBM cases.
CSF was positive by nested PCR in 17 of 17 (100%) and 18 of 27 (67%)
samples collected before and during therapy, respectively. Clinical an
d microbiological follow-up 12 weeks was available for seven patients.
PCR-positive CSF converted to M. tuberculosis DNA negative in four pa
tients that showed improvement during treatment, but it remained posit
ive in three patients who died of disseminated tuberculosis. All the C
SF samples from the non-TBM controls were negative by nested PCR. Conc
lusions: Nested PCR for detection of M. tuberculosis DNA is specific f
or diagnosis of TBM and more sensitive than conventional bacteriology.
Moreover, nested PCR could be a useful method for assessing treatment
response in AIDS patients with TBM.