SIMULATION OF FRACTOGRAMS OF FAT EMULSIONS IN POWER-PROGRAMMED SEDIMENTATION FIELD-FLOW FRACTIONATION (SDFFF)

A quasi-empirical approach to the simulation of fractograms was examin ed to verify that the elution behavior of emulsions in power-based fie ld programmed sedimentation field-flow fractionation (SdFFF) is consis tent and predictable. The approach was applied to Intralipid, a commer cial soybean emulsion and to an investigational medium chain triglycer ide emulsion. The simulations predicted the fractograms that were obta ined under various conditions of field strength, field decay and veloc ity of the suspending fluid, using distribution parameters obtained fr om one preliminary measurement of size distribution profile. Predicted fractograms were compared to experimental ones, under various fractio nating powers. Good agreement was observed in most cases, in which int erference of the secondary relaxation effects was not effective. The a greement confirmed the applicability of the approach to emulsions and that the simulations can be used instead of actual experiments for the optimization of their characterization by power-programmed SdFFF.