PRODUCTION OF IMMUNOGLOBULINS BY HUMAN SIGD(-) HUMAN BLOOD B-LYMPHOCYTES IN RESPONSE TO STIMULATION WITH ACTIVATED T-CELLS AND AGONISTIC ANTIBODIES - EFFECT OF IL-10, IL-2 AND MODE OF ACTIVATION OF T-CELLS() AND SIGD()
Nr. Ling et al., PRODUCTION OF IMMUNOGLOBULINS BY HUMAN SIGD(-) HUMAN BLOOD B-LYMPHOCYTES IN RESPONSE TO STIMULATION WITH ACTIVATED T-CELLS AND AGONISTIC ANTIBODIES - EFFECT OF IL-10, IL-2 AND MODE OF ACTIVATION OF T-CELLS() AND SIGD(), Clinical and experimental immunology, 101(2), 1995, pp. 369-375
Production of IgM, IgG and IgA was induced from human blood B lymphocy
tes by culturing with a CD40 MoAb and IL-2 for 9 days. Replacement of
IL-2 by IL-10 markedly enhanced production of all three isotypes. High
levels of immunoglobulin production also occurred when activated irra
diated autologous T cells replaced the CD40 MoAb, and when IL-10 repla
ced IL-2 in these cultures a spectacular increase in IgG production oc
curred. The effectiveness of the T cell stimulus depended on the mode
of purification of the T cells and the nature of the stimulant used to
activate them. Differences in the kinetics and level of expression of
CD40L on the various T cell preparations were observed, but did not a
ccount for variations in immunoglobulin-inducing efficiency. Immunoglo
bulin production from sIgD(+) and sIgD(-) B cells was investigated. Ig
G and IgA were found in sIgD(+) cultures, indicating that some isotype
switching had occurred, but the major part of the IgG and IgA secrete
d was from cells already committed to these isotypes. Anti-IgD or anti
-IgM MoAbs enhanced the proliferation of B cells induced by anti-CD40
antibody, but immunoglobulin production was not enhanced. Factors affe
cting the balance of proliferation and differentiation are discussed.