A. Machordom et al., PHYLOGENY AND EVOLUTION OF THE GENUS BARBUS IN THE IBERIAN PENINSULA AS REVEALED BY ALLOZYME ELECTROPHORESIS, Journal of Fish Biology, 47(2), 1995, pp. 211-236
Variation of 14 enzyme systems encoded by 31 presumptive loci in diffe
rent barbel species was studied using starch gel electrophoresis. Eigh
teen samples representing 11 Barbus tetraploid taxa were taken, includ
ing 10 populations from the Iberian Peninsula, six from other parts of
western and southern Europe, one from northern Africa and one diploid
species as outgroup from Asia Minor. The genetic analysis reassessed
of the taxonomic status of the Iberian barbels into two distinct speci
es groups. The first group included B. bocagei, B. coiniza, B. graelis
ii, B. guiraonis, B. microcephalus, and B, sclateri, that aligned with
B. callensis from northern Africa and with B. apoensis from Asia Mino
r; the other group included B. haasi and B. meridionalis that was rela
ted to the European species, B, barbus, B. plebejus and B. peloponnesi
us. These groups are probably not monophyletic. It is suggested that t
he isolation of the Iberian Peninsula from Europe since the Oligocene-
Miocene may explain the genetic affinities of the Iberian barbels with
those of North African rather than with the European group. (C) 1995
The Fisheries Society of the British isles