OPTIMIZATION OF THE DETERMINATION OF AMINO-ACIDS IN PARENTERAL SOLUTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN DERIVATIZATION USING 9-FLUORENYLMETHYL CHLOROFORMATE
B. Carratu et al., OPTIMIZATION OF THE DETERMINATION OF AMINO-ACIDS IN PARENTERAL SOLUTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN DERIVATIZATION USING 9-FLUORENYLMETHYL CHLOROFORMATE, Journal of chromatography, 708(2), 1995, pp. 203-208
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An automatic precolumn derivatization procedure is presented for the d
etermination of amino acids in parenteral solutions by reversed-phase
high-performance liquid chromatography. The fluorenylmethyl chloroform
ate was used as the reagent for derivatization. The optimum conditions
for the derivatization reaction, separation and detection were invest
igated from reproducibility and linearity data. This method has the ad
vantage of automatic precolumn derivatization, a shorter analysis time
with optimum separation and high reproducibility of retention times a
nd peak areas for all amino acids with both UV and fluorescence detect
ion.