CHIRAL SEPARATIONS OF BETA-BLOCKING DRUG SUBSTANCES USING CHIRAL STATIONARY PHASES

Three chiral stationary phases were evaluated for their usefulness in determinations of optical purity and separations of P-blocking drug su bstances. The phases were a modified cellulose phase (Chiralcel OD), a n immobilized protein (AGP) and a P-cyclodextrin phase (Cyclobond I). These phases were evaluated concerning their practical analytical use for the determination of the enantiomeric purity of non-racemic P-bloc king substances. The best phase proved to be Chiralcel OD, which provi ded excellent separations of almost all P-blocking substances tested a nd rugged determinations performed with satisfactorily low detection L imits. Similar results could be obtained with AGP, but this did not se em to be sufficiently rugged for routine analysis. The P-cyclodextrin phase resulted in poor chiral separations of most P-blocking substance s and the usefulness of this phase for analytical purposes seems limit ed.