DIAGNOSIS OF BRUCELLA-OVIS INFECTION OF RAMS WITH AN ELISA USING PROTEIN-G AS CONJUGATE

Citation
A. Ficapal et al., DIAGNOSIS OF BRUCELLA-OVIS INFECTION OF RAMS WITH AN ELISA USING PROTEIN-G AS CONJUGATE, Veterinary record, 137(6), 1995, pp. 145-147
Citations number
15
Categorie Soggetti
Veterinary Sciences
Journal title
ISSN journal
00424900
Volume
137
Issue
6
Year of publication
1995
Pages
145 - 147
Database
ISI
SICI code
0042-4900(1995)137:6<145:DOBIOR>2.0.ZU;2-F
Abstract
The complement fixation and gel diffusion tests for Brucella ovis ram epididymitis were compared with an indirect ELISA using antigens from B ovis extracted in hot saline, rough B ovis lipopolysaccharide or a c ytosolic fraction of rough B melitensis 115, and commercial anti-IgG ( heavy and light chain specificity) or protein G conjugates, None of th e antigens and conjugates used in the ELISA gave better results in ter ms of sensitivity and specificity than the complement fixation or the gel diffusion tests with the sera of 41 rams naturally infected with B ovis, 17 rams inoculated conjunctivally with B ovis and 53 Brucella-f ree rams, The protein G conjugate significantly reduced the background reactivity of the sera from the Brucella-free rams but did not improv e the sensitivity of the ELISA With the anti-IgG conjugate.