SIMULTANEOUS DETERMINATION OF AMPHETAMINE, METHAMPHETAMINE, 3,4-METHYLENEDIOXYAMPHETAMINE AND 3,4-METHYLENEDIOXYMETHAMPHETAMINE IN HUMAN HAIR BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A procedure is presented for the simultaneous identification and quant ification of amphetamine (AP), methamphetamine (MA), methylenedioxyamp hetamine (MDA) and methylenedioxymethamphetamine (MDMA) in human hair. The method involves decontamination of hair with dichloromethane and warm water, heat-alkaline hydrolysis in the presence of deuterated int ernal standards, liquid-liquid extraction and gas chromatography-mass spectrometry after derivatization with pentafluoropropionic anhydride- pentafluoropropanol. The limit of detection for AP, MA and MDA was 0.0 5 ng/mg using a 50-mg hair sample; for MDMA it was 0.1 ng/mg. Coeffici ents of variation ranged from 7 to 18%. This assay has been successful ly utilized in the evaluation of the deposition of the drugs in hair o btained from various parts of the anatomy of a stimulant abuser.