CAPILLARY ZONE ELECTROPHORESIS OF OLIGOSACCHARIDES DERIVATIZED WITH N-(4-AMINOBENZOYL)-L-GLUTAMIC ACID FOR ULTRAVIOLET ABSORBENCY DETECTION

Authors
PLOCEK J NOVOTNY MV
Citation
J. Plocek et Mv. Novotny, CAPILLARY ZONE ELECTROPHORESIS OF OLIGOSACCHARIDES DERIVATIZED WITH N-(4-AMINOBENZOYL)-L-GLUTAMIC ACID FOR ULTRAVIOLET ABSORBENCY DETECTION, Journal of chromatography, 757(1-2), 1997, pp. 215-223
Citations number
27
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
757
Issue
1-2
Year of publication
1997
Pages
215 - 223
Database
ISI
SICI code
Abstract
A charged and strongly UV-absorbing tag, N-(4-aminobenzoyl)-L-glutamic acid) (ABG), was coupled to oligosaccharides by reductive amination u nder mild conditions. The effectiveness of ABG as a derivatization age nt is shown through the separation of isomaltooligosaccharides from a dextran hydrolysate. The minimum detectable quantities in the subpicom ole range are demonstrated.