Rj. Caimi et Jt. Brenna, QUANTITATIVE-EVALUATION OF CARBON ISOTOPIC FRACTIONATION DURING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 757(1-2), 1997, pp. 307-310
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The fractionation of C-13 during low-performance preparative LC and hi
gh-performance LC is reported quantitatively for methyl palmitate and
using high-precision isotope ratio mass spectrometry (IR-MS). For both
preparative and high-performance analytical columns, C-13 enrichment
is about 7 parts per thousand greater than the parent starting materia
l, drops sharply in the first section of the peak and then settles to
a value about 1 parts per thousand below that of the starting material
. Recycling over a single HPLC column did not induce greater fractiona
tion. These results emphasize the importance of quantitative peak coll
ection for high-precision IR-MS studies, particularly the first part o
f the peak where the isotope ratio changes rapidly.