A SIMPLE AND SENSITIVE DNA HYBRIDIZATION ASSAY USED FOR THE ROUTINE DIAGNOSIS OF HUMAN PARVOVIRUS B19 INFECTION

Authors
HICKS KE BEARD S COHEN BJ CLEWLEY JP
Citation
Ke. Hicks et al., A SIMPLE AND SENSITIVE DNA HYBRIDIZATION ASSAY USED FOR THE ROUTINE DIAGNOSIS OF HUMAN PARVOVIRUS B19 INFECTION, Journal of clinical microbiology, 33(9), 1995, pp. 2473-2475
Citations number
21
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
9
Year of publication
1995
Pages
2473 - 2475
Database
ISI
SICI code
0095-1137(1995)33:9<2473:ASASDH>2.0.ZU;2-4
Abstract
A dot blot hybridization assay for parvovirus B19 diagnosis was develo ped by using a PCR-generated probe, digoxigenin labelling, and chemilu minescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised f or routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samp les collected during 1994 and 1995 are given.