IMMUNOLOGICAL DETECTION OF PENICILLIN-BINDING PROTEIN-2' OF METHICILLIN-RESISTANT STAPHYLOCOCCI BY USING MONOCLONAL-ANTIBODIES PREPARED FROM SYNTHETIC PEPTIDES

Two synthetic peptides 31 and 32 amino acids in length were prepared a s deduced from a known amino acid sequence of penicillin-binding prote in 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two mono clonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibo dies to PBP2' of a methicillin-resistant S. aureus strain. An immunora diometric assay was developed by using these antibodies for simple det ection of PBP2'.