SIGNIFICANCE OF URINARY FIBRIN FIBRINOGEN DEGRADATION PRODUCTS (FDP) D-DIMER MEASURED BY A HIGHLY SENSITIVE ELISA METHOD WITH A NEW MONOCLONAL-ANTIBODY (D-D E72) IN VARIOUS RENAL DISEASES

To diagnose the abnormalities of coagulation-fibrinolysis in various r enal diseases, we developed a new monoclonal antibody (D-D E72) agains t fibrin/fibrinogen degradation products D-dimer (FDP D-dimer) and est ablished a highly sensitive enzyme-linked immunosorbent assay (ELISA) for its measurement. FDP D-dimer was assessed in 102 patients with var ious renal diseases, and the following results were obtained: 1. The m ean level of urinary FPD D-dimer in 32 normal controls was 0.69 +/- 0. 60 ng/ml (mean +/- SD). 2. The level of urinary FDP D-dimer was signif icantly higher in primary nephrotic syndrome group (NS), chronic renal failure group (CRF) and in the group of diabetic nephropathy (DM) tha n in the control group. However, no difference was observed in the lev el of urinary FDP D-dimer between non-nephrotic chronic glomerulonephr itis group (CGN) and control group. 3. No significant correlation was revealed between D-dimer and urinary protein in CGN and NS groups. The se results suggest that in addition to plasma filtration the urinary F DP D-dimer in NS, CRF and DM may be also related to abnormalities of s econdary fibrinolysis in intra-glomerular fibrin deposits.