AN INCREASED FREQUENCY OF IGE-PRODUCING B-CELL PRECURSORS CONTRIBUTESTO THE ELEVATED LEVELS OF PLASMA IGE IN ATOPIC SUBJECTS

Background The production of specific IgE, which underlies the allergi c response, may be a normal correlate of the immune response to a cert ain class of antigen (allergens), or could represent a unique response driven by regulatory signals that are absent in non-allergic individu als. If atopic subjects do possess a regulatory environment favoring I gE production, they may display not only allergen-specific IgE, but al so higher levels of total IgE and higher frequencies of IgE-producing B lymphocytes. Objective To address the contribution of antibody-produ cing cell number to the circulating IgE titre in atopic vs non-atopic subjects. Methods Frequency determination by limiting dilution of EBV transformants and Poisson distribution analysis. Titres of total and a llergen-specific IgM, IgG, and IgE by specific ELISA. Results In contr ast to findings reported by others, the atopic subjects had a signific antly higher frequency of IgE-producing B cells than non-atopics (0.79 % of total Ig-producing cells, as compared with 0.17% for the control group; P < 0.01), suggesting that one factor contributing to the high plasma IgE titres in atopic subjects is the high frequency of B lympho cytes with the potential to produce IgE. Although only the atopic subj ects produced allergen-specific IgE, the frequency of specific IgE-pro ducing B cells was undetectable in both groups. Conclusion Atopic subj ects have higher frequencies of IgE-producing B cell precursors than n on-atopics. A correlation exists between IgE-producing B cell frequenc y and levels of circulating IgE.