GENETIC ASSOCIATION BETWEEN CHROMOSOME-8 MICROSATELLITE (MS8-134) ANDWERNER SYNDROME (WRN) - CHROMOSOME MICRODISSECTION AND HOMOZYGOSITY MAPPING

Werner syndrome (WRN) is an autosomal recessive disorder characterized by premature aging that has been mapped to the short arm of chromosom e 8, 8p11.2-p12. To refine the genetic map around the WRN region, we h ave isolated eight microsatellites for this region from a microdissect ion library. We typed members of Japanese families with WRN on the bas is of homozygosity mapping analysis. There was no obligate recombinati on between the WRN locus and microsatellite clone, MS8-134 (D8S1055). The maximum lod score was 20.28 at theta = 0.00. Alleles for MS8-134 s howed association with WRN in a case-control study (OR = 3.55, 95% CI 1.56-8.07, P < 0.01). Such microsatellites hom a microdissection Libra ry of the definite chromosome region may be useful for positional clon ing of the WRN gene. (C) 1995 Academic Press, Inc.