MAPPING OF THE INSULIN PROMOTER FACTOR-1 GENE (IPF1) TO DISTAL MOUSE CHROMOSOME-5

We have identified sequence variants in the 3' untranslated region of the insulin promoter factor 1 (Ipf1) cDNA sequence in mice and used a PCR-based oligonucleotide hybridization assay to map the Ipf1 gene to distal mouse chromosome (Chr) 5. An identical 12-bp insertion in the 3 ' untranslated region of the mouse Ipf1 gene sequence is present in Mu s spretus and Mus m. musculus Czech II mice, two feral strains used fo r meiotic mapping by backcross analysis. The human IPF1 gene homolog w ould be expected to map to either chromosome 13q12-q14 or 7pter-q21 ba sed on homology of synteny of other loci in this region of mouse Chr 5 . Given its genetic map position, the Ipf1 gene may be part of a casse tte of homeodomain-containing transcription factors involved in the de velopment of the pancreas and other foregut-derived organs. (C) 1995 A cademic Press, Inc.