Sections of flash-frozen saliva particle mixture were examined by tran
smission electron microscopy. The saliva samples used consisted of 40%
by volume parotid and 60% by volume submandibular sublingual fraction
s. One mL of the saliva was mixed with 0.1 mt of either hydrophilic or
hydrophobic particles of 0.9 mu m diameter. The micrographs of the sa
liva and hydrophilic particles mixes showed the multiple spherical par
ticles in the salivary network structures. Micrographs of the saliva a
nd hydrophilic particle mixes showed the presence of few such objects;
when observed, the particles were located only in the electron dense
part of the salivary structures.