ULTRASTRUCTURAL COLOCALIZATION OF VIMENTIN AND CYTOKERATIN IN VISCERAL GLOMERULAR EPITHELIAL-CELLS OF DOGS WITH GLOMERULONEPHRITIS

The expression of cytokeratin and vimentin was studied in the glomerul ar epithelial cells of canine kidneys with and without glomerular abno rmalities. Using ultrastructural, immunogold single and double labelli ng techniques, cytokeratin and vimentin were found together in the vis ceral glomerular epithelial cells (vGECs) of abnormal kidneys. In norm al kidneys, the vGECs expressed only vimentin, and cytokeratin was fou nd exclusively in parietal glomerular epithelial cells (pGECs). These results confirm previous findings in the same animals, obtained by imm unohistological staining techniques.