MULTIPLE TOXICITY OF 3 METALS, TRIBUTYLTIN CHLORIDE, DIBUTYLTIN DICHLORIDE AND MONOBUTYLTIN TRICHLORIDE, USING THE MARINE BACTERIUM VIBRIO-HARVEYI AS THE TEST ORGANSM

Vibrio harveyi, a bioluminescent marine bacterium, was used in a direc t assay procedure to evaluate chemical mixtures of tributyltin chlorid e, dibutyltin dichloride and monobutyltin trichloride in a water envir onment. combined toxicity was evaluated using an isobole plot, isobolo gram method, additive index calculation and statistical test. Results suggest that these mixtures are statistically additive using the isobo le and isobologram, less than additive by the additive index calculati on and significantly different when various combinations are compared. These study results suggest that solution compositions of different c oncentrations have descriptively dissimilar effects on the test organi sm although these differences do not appear to be statistically differ ent. The applicability of the V. harveyi toxicity test for mixture stu dies was evaluated.