CLONING AND CHARACTERIZATION OF CHINESE-HAMSTER P53 CDNA

We have cloned and sequenced Chinese hamster p53 cDNA and have compare d the p53 sequence in different Chinese hamster cell lines to several relevant phenotypes. Our results indicate that a mutation in CHO cells that changes Thr(211) to Lys(211) abrogates the ability to arrest in G(1) and apparently renders cells capable of amplifying DNA. However, this mutation has no effect on the G(2) checkpoint or on acute down-re gulation of DNA replication after a radiation challenge.