Jc. Bagshaw et Ma. Buckholt, A NOVEL SATELLITE MICROSATELLITE COMBINATION IN THE GENOME OF THE MARINE SHRIMP, PENAEUS-VANNAMEI/, Gene, 184(2), 1997, pp. 211-214
In our studies of repeated sequences in the genome of the marine shrim
p, Penaeus vannamei (Pv), we have discovered a novel combination of se
quence elements. We inserted restriction fragments of genomic DNA into
a plasmid vector and screened for recombinant plasmids containing rep
eated sequences. Ten of the resulting isolates contained representativ
es of the same repeated element, a satellite sequence present in one o
r more blocks of tandemly repeated units. The cloned repeat units rang
e in size from 139 to 188 bp. Embedded within each cloned repeat unit
are 6-15 copies of a tandemly repeated pentanucleotide microsatellite.
The genome of Pv contains approx. 1 000 000 copies of this satellite/
microsatellite unit. Sequences that cross-hybridize strongly with this
structure were found in the genomes of lobster and crayfish, but not
in other species of the genus Penaeus.