EFFECTS OF BUTYLATED HYDROXYANISOLE, BUTYLATED HYDROXYTOLUENE AND TERTIARY BUTYLHYDROQUINONE ON GROWTH AND LUTEOSKYRIN PRODUCTION BY PENICILLIUM-ISLANDICUM

Butylated hydroxyanisole (BHA), Butylated hydroxytoluene (BHT) and ter tiary butylhydroquinone (TBHQ) alone in cultural media were tested for the inhibition of growth and luteoskyrin production by two toxigenic strains of Penicillium islandicum UST-11 and P, islandicum HLT-6. In p otato dextrose agar, the concentrations of BHA and TBHQ from 0.2 mg/di sc, BHT from 5.0 mg/disc did affect the growth of both tested strains, but the initial concentrations of these antioxidants to reduced luteo skyrin production by UST-11 strain were BHA 0.5 mg/disc, BHT 1.0 mg/di sc and TBHQ 0.4 mg/disc, while for HLT-6, BHA 0.4 mg/disc, BHT and TBH Q were 0.2 mg/disc, respectively. In grainy and powdery rice media, th e effects of BHA, BHT and TBHQ on luteoskyrin production by P. islandi cum UST-11 and HLT-6 were clearly demonstrated. The efficiency of the inhibitory effect was not only closely related to the concentration of antioxidants, but also completely inhibited the luteoskyrin productio n at a concentration of 200 mg/kg or higher. Also, the antioxidants at a concentration higher than 20 mg/kg reduced significantly the growth and luteoskyrin production by both strains of P. islandicum.