MOLECULAR-CLONING AND CHROMOSOMAL MAPPING OF A NOVEL HUMAN GENE, CHEMR1, EXPRESSED IN T-LYMPHOCYTES AND POLYMORPHONUCLEAR CELLS AND ENCODING A PUTATIVE CHEMOKINE RECEPTOR
M. Samson et al., MOLECULAR-CLONING AND CHROMOSOMAL MAPPING OF A NOVEL HUMAN GENE, CHEMR1, EXPRESSED IN T-LYMPHOCYTES AND POLYMORPHONUCLEAR CELLS AND ENCODING A PUTATIVE CHEMOKINE RECEPTOR, European Journal of Immunology, 26(12), 1996, pp. 3021-3028
We describe the cloning of a human gene, named ChemR1, encoding a new
putative chemokine receptor sharing 48% identity with CC-chemokine rec
eptor (CCR)4 and 44% identity with CCR1. It displays four extracellula
r cysteines that are conserved among all other chemokine receptors. Ch
emR1 transcripts were detected by Northern blotting in the T lymphobla
stic cell lines Jurkat and MOLT-4, but not in the pre-B lymphoblastic
cell line JM-1. ChemR1 receptor transcripts were also detected by reve
rse transcription and polymerase chain reaction analysis in unstimulat
ed CD4(+) and CD8(+) T cells and polymorphonuclear cells prepared from
peripheral blood. The chromosomal localization was performed by radia
tion hybrid mapping and testing of a panel of yeast artificial chromos
ome clones. This allowed the assignment of the ChemR1 receptor gene to
the p21.3-24 region of human chromosome 3, in close proximity with th
e functionally characterized CCR. Future work is required to identify
the ligand(s) of this new chemokine receptor and to define its role in
the recruitment of white blood cell populations.