COMPARATIVE INVESTIGATION OF THE TOPOGRAP HY OF PROTEINS OF PHOTOSYNTHETIC APPARATUS OF RHODOPSEUDOMONAS-PALUSTRIS, STRAIN AB GROWN AT VARIOUS LIGHT-INTENSITY

Using TNBS and antibodies, we studied the protein topography at variou s sites of the membranes, isolated from Rhodopseudomonas palustris cel ls, strain AB, which were grown at 300 and 30000 lux. It was shown tha t at the cytoplasmic side of the membrane, the marker was incorporated mainly in the polypeptides of light harvesting complexes and H-subuni ts of the reaction center independently on light intensity. At the per iplasmic side of the membrane, the marker was found in the antenna pro teins, in the subunits of the reaction center in lamellae in the cells cultivated at low light intensity, and in the antenna proteins in lam ellae in cells cultivated al high light intensity.