ACTION OF LEUKOTRIENE B4 IN HUMAN GRANULOSA-CELLS IN-VITRO

Leukotriene B4 directly enhanced progesterone release from superfused human granulosa cells. This secretory effect was observed in concentra tions from 10-(12) to 10(-10) M. Lower and higher concentrations faile d to affect progesterone release. When we analysed the high performanc e liquid chromatography profile of supernatant from human granulosa ce ll cultures, we detected a leukotriene B4 peak. In conclusion, these d ata support the hypothesis that leukotriene B4 may participate in the intracellular mechanism of progesterone release in human granulosa cel ls.