HISTOLOGIC EFFECTS OF DIFFERENT SUTURE MATERIALS IN MICROSURGICAL ANASTOMOSIS OF THE RAT UTERINE HORN

OBJECTIVE: To evaluate the histologic effects and permeability rate of different suture materials in microsurgical anastomosis of the left u terine horn in Wistar rats. STUDY DESIGN: In this histologic study, 81 female Wistar rats were used. A transverse section and anastomosis we re performed using three surgical techniques. The rats were divided in to 15 experimental groups based on evaluation of three variations in m icrosurgical technique: (1) one-layer transmucosal anastomosis, (2) on e-layer extramucosal anastomosis, and (3) two-layer transmucosal anast omosis. With each technique, five structure materials were used: 7/0 p olyglycolic acid, 6/0 polyglactin, 7/0 polydioxanone, 7/0 silk and 7/0 polypropylene. RESULTS: The permeability rate was 86%. Polydioxanone persisted in 41% of eases at 90 days. Abnormal proliferation of mucosa l epithelium was verified in 10% of eases. Polypropylene was responsib le for the majority of foreign-body granulomas. Polydioxanone produced the smallest inflammatory reaction. CONCLUSION: The type of suture ma terial did nor influence the permeability rate. Use of nonabsorbable s uture material altered the histologic structure the most and produced the greatest tissue reaction. The best results were obtained with poly dioxanone. Monofilial sutures gave vise to fewer structural alteration s than did multifilament sutures.