EFFECT OF AMYGDALOID KINDLING ON THE CONTENT AND RELEASE OF AMINO-ACIDS FROM THE AMYGDALOID COMPLEX - IN-VIVO AND IN-VITRO STUDIES

The tissue content and the interstitial fluid levels of glutamate, asp artate, GABA, glutamine, glycine, and serine were studied in amygdaloi d-kindled rat brain, Interstitial levels were studied in vivo before a nd during stage 5 full limbic seizures using microdialysis. Slices of amygdala from kindled and sham-operated animals were used to study bas eline and KCl-evoked release in vitro. The contents of these amino aci ds were measured in slices of amygdala, hippocampus, and cerebral cort ex from kindled and sham-operated animals, Kindled brains showed two- to threefold higher levels of glutamate, aspartate, and GABA and 12-fo ld higher levels of glutamine than sham-operated controls. Correlating with this, interstitial fluid levels of glutamate were two- to threef old higher from kindled amygdala than from control both in vivo (micro dialysis) and in vitro (superfusion). GABA levels in interstitial flui d from kindled amygdala were reduced by 67% compared with control amyg dala.