ACTIVITY OF C-BETA-1-]6(GLCNAC-BETA-1-]2)MAN-ALPHA-1-]R[GLCNAC TO MAN]BETA-1-]4N-ACETYLGLUCOSAMINYLTRANSFERASE-VI (GNT-VI) FROM THE OVARIESOF ORYZIAS-LATIPES (MEDAKA FISH)

Authors
TAGUCHI T KITAJIMA K INOUE S INOUE Y YANG JM SCHACHTER H BROCKHAUSEN I
Citation
T. Taguchi et al., ACTIVITY OF C-BETA-1-]6(GLCNAC-BETA-1-]2)MAN-ALPHA-1-]R[GLCNAC TO MAN]BETA-1-]4N-ACETYLGLUCOSAMINYLTRANSFERASE-VI (GNT-VI) FROM THE OVARIESOF ORYZIAS-LATIPES (MEDAKA FISH), Biochemical and biophysical research communications, 230(3), 1997, pp. 533-536
Citations number
30
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
230
Issue
3
Year of publication
1997
Pages
533 - 536
Database
ISI
SICI code
0006-291X(1997)230:3<533:AOCTM>2.0.ZU;2-Z
Abstract
UDP-GlcNAc:GlcNAc beta 1-->(GlcNAc beta 1-->2)Man alpha 1-R[GlcNAc to Man]beta 1-->4N-acetylglucosaminyltransferase VI (GnT VI) activity was shown to be present in crude homogenates of Medaka fish (Oryzias lati pes) ovaries using UDP-[C-14]GlcNAc and synthetic GlcNAc beta 1-->6 (G lcNAc beta 1-->2)Man alpha 1-->6Glc beta 1-->octyl as substrates. Char acterization of this activity showed a pH optimum at about pH 7.0 and an absolute requirement for divalent cations. The optimum concentratio n of Mn2+ was at about 25 mM. This finding is the first report on GnT VI activity in fish; the enzyme has previously been described only in avian tissues. (C) 1997 Academic Press.