A KINETIC-STUDY ON THE HYDROLYSIS OF FISH -PROTEIN BY ACID PROTEASE

Fish protein was solubilized by an acid protease in a batch reactor. N on-salted pollack frozen surimi was used as substrate. Parametric stud ies were carried out to determine the effects of temperature, pH, init ial substrate concentration and enzyme concentration on the kinetics o f solubilization. The reaction rate equation was presented from the ki netic model which was based on Michaelis-Menten type kinetics, taking account of product inhibition. Furthermore, a simple rate equation was derived by simplifying the above equation. Parameters in the rate equ ation were estimated by using observed data. The experimental data wer e in good agreement with the prediction calculated from the equations.