T. Kometani et al., SYNTHESIS OF HESPERIDIN GLYCOSIDES BY CYCLODEXTRIN GLUCANOTRANSFERASEAND STABILIZATION OF THE NATURAL PIGMENTS, J JPN SOC F, 42(5), 1995, pp. 376-382
Citations number
NO
Categorie Soggetti
Food Science & Tenology
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
An improved method for the synthesis of hesperidin glycosides was deve
loped using both beta-cyclodextrin (beta-CD) and alpha-rhamnosidase tr
eatments. The reaction, which contained 0.5% (8.2 mM) hesperidin as an
acceptor, 5% soluble starch as a donor and 2 units/ml of cyclodextrin
glucanotransferase (CGTase) from an alkalophilic Bacillus species was
incubated at 40 degrees C and pH 10 for 16 h, was used as the standar
d reaction. During the transglycosylation reaction, both beta-CD and a
n alkaline pH were very effective for solubilizing hesperidin, and the
amount of hesperidin glycosides formed by CGTase with 5% B-CD as a do
nor at pH 10 was about 50 times greater than the amount formed with so
luble starch at pH 5. During the purification steps, alpha-rhamnosidas
e hydrolyzed the unreacted hesperidin into lower molecular weight and
more hydrophobic compounds (hesperetin-7-glucoside), but it did not hy
drolyze the hesperidin glycosides. Therefore, hesperidin glycosides we
re easily separated from the unreacted hesperidin using Sephadex LH-20
. Various kinds of natural pigments were stabilized against ultraviole
t radiation using the prepared hesperidin glycosides.