STUDIES ON MODIFIED ATMOSPHERE PACKAGING OF THIN SLICED BEEF .2. EFFECT OF MODIFIED ATMOSPHERE PACKAGING ON MYOFIBRILLAR FRAGMENTATION, EXTRACTABILITY OF MUSCLE PROTEIN AND LDH ACTIVITY OF THIN SLICED BEEF
M. Muguruma et al., STUDIES ON MODIFIED ATMOSPHERE PACKAGING OF THIN SLICED BEEF .2. EFFECT OF MODIFIED ATMOSPHERE PACKAGING ON MYOFIBRILLAR FRAGMENTATION, EXTRACTABILITY OF MUSCLE PROTEIN AND LDH ACTIVITY OF THIN SLICED BEEF, J JPN SOC F, 42(7), 1995, pp. 505-511
Citations number
25
Categorie Soggetti
Food Science & Tenology
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
The objective of our study was to investigate the effect of modified a
tmosphere packaging on myofibrillar fragmentation, extractability of m
uscle protein and LDH activity oi thin sliced beef. The sample was pac
kaged in atmospheres of: (a) air without gas flush treatment (control)
, (b) 20% CO2 + 80% O-2 after vacuum treatment, (c) 20% CO2 + 80% O-2
after being flushed with the gas of the same composition and then was
stored at 5 degrees C for 2, 4, 6, and 8 days. The degree of myofibril
lar fragmentation of all samples gradually increased during storage. T
he rate of myofibrillar fragmentation increased in order of control >(
b)>(c). The extractability of water- and salt-soluble muscle proteins
of all samples decreased during storage. SDS-PAGE patterns of proteins
extracted with GS-solution from thin sliced beef stored under differe
nt conditions exhibited a decrease in salt-soluble protein due primari
ly to the decrease in the extractability of myosin molecules. LDH acti
vity of the control decreased during storage, but the samples packaged
in modified atmospheres maintained their LDH activity, particularly t
he (c) sample did. These results suggest that samples packaged in modi
fied atmospheres retain biological activity even after 8 days of stora
ge.