DIRECT DETERMINATION OF DIPYRIDAMOLE IN SERUM

A new method for the determination of dipyridamole in serum for concen trations between 10 and 100 ng ml(-1) by means of matrix isopotential synchronous fluorescence spectrometry and derivative techniques is pro posed. This new method is useful for the determination of compounds in samples with unknown background fluorescence, such as dipyridamole in serum, without the need for tedious preseparation. The determination was performed in an ethanol/water medium (15% v/v) at a pH value of 10 .0, provided by adding an ammonium chloride/ammonia buffer solution. S ince dipyridamole is a stimulant which is very much used like a doping substance in sport, the method was successfully applied to the determ ination of dipyridamole in serum. Better sensitivity and repeatability are achieved for these matrices than with the fluorometric ones descr ibed in the literature. An exhaustive statistical analysis has been de veloped for all calibration graphs. This treatment includes robust reg ression such as least median of squares which also detects outliers an d leverage points. The overall least-squares regression has been appli ed to find the more exact straight line which fits the experimental da ta. The error propagation has been considered to calculate the detecti on limit, determination limit, and the repeatability of the method. (C ) 1997 Academic Press.