DETERMINATION OF 2-KETO-3-DEOXYOCTULOSONIC ACID (KDO) WITH HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY (HPAEC) - SURVEY OF STABILITY OF KDO AND OPTIMAL HYDROLYTIC CONDITIONS

Authors
KIANG J SZU SSC WANG LX TANG M LEE YC
Citation
J. Kiang et al., DETERMINATION OF 2-KETO-3-DEOXYOCTULOSONIC ACID (KDO) WITH HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY (HPAEC) - SURVEY OF STABILITY OF KDO AND OPTIMAL HYDROLYTIC CONDITIONS, Analytical biochemistry, 245(1), 1997, pp. 97-101
Citations number
15
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
245
Issue
1
Year of publication
1997
Pages
97 - 101
Database
ISI
SICI code
0003-2697(1997)245:1<97:DO2A(W>2.0.ZU;2-7
Abstract
A new method for 2-keto-3-deoxy-octulosonic acid (KDO) determination u sing high-performance anion-exchange chromatography was developed. KDO is well separated from Neu5Ac, and the response was linear from 20 pm ol to 5 nmol. The method was used to examine the stability of KDO unde r various hydrolytic conditions and to survey optimal hydrolytic condi tions for release of KDO. (C) 1997 Academic Press.