PRESENCE OF MULTIPLE AMYLASES ENCODED BY INDEPENDENT GENES IN CELLULOMONAS SP NCIM-2353

Four genomic fragments encoding independent amylases from Cellulomonas sp. NCIM 2353 were cloned in Escherischia coil DH10B, using plasmid B luescript. The enzyme encoded by these clones could be induced in the presence of starch, without IPTG. On zymogram amylase activity stainin g, two of these clones were seen to produce independent enzymes, corre sponding to the Cellulomonas amylases. The restriction digestion profi les confirmed that these four DNA fragments are situated at different locations on the Cellulomonas genome. Some of the clones also harbored xylanase activity.