APPLICATION OF THE PCLODF13 BACTERIOCIN RELEASE PROTEIN IN THE RELEASE OF HETEROLOGOUS PROTEINS BY ESCHERICHIA-COLI - PRODUCTION OF PLANT ALPHA-GALACTOSIDASE

Authors
VANDERWAL F TENHAGEN CM OUDEGA B LUIRINK J
Citation
F. Vanderwal et al., APPLICATION OF THE PCLODF13 BACTERIOCIN RELEASE PROTEIN IN THE RELEASE OF HETEROLOGOUS PROTEINS BY ESCHERICHIA-COLI - PRODUCTION OF PLANT ALPHA-GALACTOSIDASE, Biotechnology letters, 17(8), 1995, pp. 815-820
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
8
Year of publication
1995
Pages
815 - 820
Database
ISI
SICI code
0141-5492(1995)17:8<815:AOTPBR>2.0.ZU;2-D
Abstract
The bacteriocin release protein (BRP) mediated release of several euka ryotic proteins was studied in Escherichia coil. The genes for the pla nt proteins a-galactosidase and thaumatin, and for the mammalian prote ins prochymosine and prophospholipase A(2), were cloned behind the Omp A signal peptide. The alpha-galactosidase was expressed and secreted i nto the periplasm. Prochymosine and thaumatin were poorly expressed. R elease experiments showed that about 5 mg/l of alpha-galactosidase was excreted into the extracellular medium upon induction of the BRP.