TRANSFER OF A BENOMYL RESISTANCE MARKER BY HEAT-INACTIVATED TRICHODERMA-REESEI PROTOPLASTS

Authors
MEZA V MORENO P TENGERDY RP GUTIERREZCORREA M
Citation
V. Meza et al., TRANSFER OF A BENOMYL RESISTANCE MARKER BY HEAT-INACTIVATED TRICHODERMA-REESEI PROTOPLASTS, Biotechnology letters, 17(8), 1995, pp. 827-832
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
8
Year of publication
1995
Pages
827 - 832
Database
ISI
SICI code
0141-5492(1995)17:8<827:TOABRM>2.0.ZU;2-C
Abstract
Protoplasts from a benomyl resistant Trichoderma reesei mutant were he at inactivated at 60 degrees C for 8 min and fused with viable protopl asts from an osmosensitive, non-sporulating T. reesei strain. Fusants recovered on 50 mu g/ml benomyl containing potato dextrose agar plates grew and sporulated well. Cellulolytic enzyme activities produced in liquid culture by selected fusants were higher than those produced by parental strains.