J. Han et al., CONCENTRATING ALKALINE SERINE-PROTEASE, SUBTILISIN, USING A TEMPERATURE-SENSITIVE HYDROGEL, Biotechnology letters, 17(8), 1995, pp. 851-852
The alkaline serine protease, subtilisin, produced by Bacillus licheni
formis was concentrated using hydrogel ultrafiltration. Separation eff
iciency at 15 degrees and 20 degrees C was 84% but decreased above 25
degrees C.