T. Ishizuka et al., SYNAPHIN - A PROTEIN ASSOCIATED WITH THE DOCKING FUSION COMPLEX IN PRESYNAPTIC TERMINALS/, Biochemical and biophysical research communications, 213(3), 1995, pp. 1107-1114
We previously identified a 19 kDa protein associated with the docking/
fusion complex involved in neurotransmitter release. A cDNA for this p
rotein was cloned from a bovine brain cDNA library using an oligonucle
otide probe based on its partial amino acid sequence. The protein (nam
ed synaphin) en coded by the cDNA is a very hydrophilic protein rich i
n glutamic acid and lysine residues. It lacks any putative transmembra
ne segments or strongly hydrophobic domains. Immunoblots with antibodi
es against synaphin detected the protein only in the nervous system am
ong the tissues examined In brain, it exists mainly in the soluble fra
ction and is scarce in synaptic vesicles. (C) 1995 Academic Press, Inc
.