EFFECT OF BUTACHLOR ON THE BIOSYNTHESIS OF LIPID, PROTEIN, AND NUCLEIC-ACID IN 3 ISOLATED PLANT-CELLS

About one half of lipid synthesis was inhibited in Chlorella (46%), to bacco leaf cell (58%), and rice embryo cell (45%) when the isolated ce ll was incubated at 70 mu m of butachlor for 4 hr. 4 thirty percent di fference of unincorporated acetate pool size between 70 mu m of butach lor treated (71%) and untreated (41%) tobacco leaf cells was found in lipid synthesis, but no significant difference was observed in that of Chlorella and rice embryo cells. Within 30 min incubation at 70 mu m of butachlor, protein synthesis was inhibited by 79, 59, and 38% for C hlorella, tobacco leaf cells, and rice embryo cells, respectively, and the inhibition was increased by 92, 95 and 85%, respectively, at 8 hr . In contrast to untreated cells, there were 69, 62, and 39% larger po ols of absorbed C-14-leucine after treatment of the three cell types w ith 70 mu m of butachlor. However, total leucine uptake showed in trea ted cells was only 52, 37, and 51%, respectively, of that in control. Butachlor inhibited RNA synthesis at 70 mu m, but at lower concentrati ons, even stimulation was found.