AUXILIARY METHOD FOR CLONAL IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS BY PROTEIN BAND PATTERN OF RELEASED PROTEINS ON SDS-POLYACRYLAMIDE GEL

Authors
SEKI K SAKURADA J MURAI M USUI A SEONG HK JITSUKAWA H MASUDA S
Citation
K. Seki et al., AUXILIARY METHOD FOR CLONAL IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS BY PROTEIN BAND PATTERN OF RELEASED PROTEINS ON SDS-POLYACRYLAMIDE GEL, Microbiology and immunology, 39(8), 1995, pp. 615-617
Citations number
15
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
39
Issue
8
Year of publication
1995
Pages
615 - 617
Database
ISI
SICI code
0385-5600(1995)39:8<615:AMFCIO>2.0.ZU;2-#
Abstract
A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surfa ce culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration proced ure prior to electrophoresis. The combined use of cellophane surface c ulture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization.